AAV Catalog

Our new Control AAVs are here!

BEC Enhancer AAVs

CNCM has developed new useful tools for delivering genetic payloads specifically to Brain Endothelia cells (BECs) for normal and diseased brain studies. These vectors specifically target brain endothelial cells (BEC) based on the cis-regulatory elements identified from single-cell epigenetic datasets. Ex vivo and in vivo characterization of BEC enhancer AAVs in wild type, Ai9 reporter, and Alzheimer’s disease mouse brains show their utility for high transduction selectivity of the brain endothelial cells with little off-target transduction in the liver. Our BEC enhancer AAVs target the brain vasculature by systemic administration and can be minimally invasive in terms of the route of administration. More information about these viruses can be found on the following publication: Specific targeting of brain endothelial cells using enhancer AAV vectors. Eric Velazquez-Rivera, et al. (Neuron, in press) 

Size per aliquot: 50uL

Serotypes offered: PHP.eB and BR1 (Additional serotypes and payloads may be available via custom AAV orders.)

Enhancer AAVs- Dr. Gordon Fishell/ Broad Institute

The CNCM has partnered up with lab of Dr. Gordon Fishell at the Broad Institute at Harvard University to produce and distribute cell-type specific enhancer AAV reagents as part of the BRAIN Initiative Armamentarium project. These enhancer AAVs enable precise genetic targeting of specific cell types across rodents, non-human primates, and other vertebrates, facilitating targeted manipulation of neural circuits. This property is crucial for advancing the understanding of normal brain function. The incorporation of gene regulatory elements for precise gene expression represents a groundbreaking advancement in the field of brain circuitry research. More information about these viruses can be found on the following publication: An enhancer-AAV toolbox to target and manipulate distinct interneuron subtypes. Elisabetta Furlanis, Min Dai, et al. Preprint

Size per aliquot: 50uL

Serotypes offered: PHP.eB or AAV2-retro (Additional serotypes and payloads may be available via custom AAV orders.)

We are pleased to collaborate with Dr. Don Arnold at USC to distribute their ATLAS AAVs. To learn more about the ATLAS AAVs, please read the preprint here. Steps to ordering the ATLAS AAVs:

• Obtain written permission from Dr. Arnold.
• Execute an MTA between USC and your organization.
• Order the ATALS AAVs via our online order form (below)
• Execute a Sales & Service (S&S) with UCI and your organization.
• Virus will be shipped once the MTA and S&S are finalized and PO# is provided.

All ATLAS AAVS come as AAV8, custom serotypes obtain permission from Dr. Arnold and are considered custom scale (0.5mL).

Our CNCM core currently offer Custom AAV virus services. We offer full service from gene synthesis/cloning, packaging to validation services. You can provide your plasmid or get one custom built to suit your needs. We offer over 150+ serotypes.

Expect a turnaround time for 6-8 weeks depending on service. Please note more complex cloning will require longer turnaround time.

After maxi prepping and packaging your DNA into your AAV serotype of choice, we purify with CsCl2 gradient ultracentrifugation to get rid of the empty vectors, titer> 1 x 10¹³ GC/ml in 0.5 ml. Titers are preformed via ddPCR.

We are pleased to work with Dr. Yulong Li to distribute their large catalog of GRAB Sensor AAVs. Pick from a wide array of options by clicking on the button below!
Note: GRAB sensors are only provided in PHP.eB serotype and 100µL aliquots.

Visit our sister core at Cal Poly Pomona for capsid controls such as CAP-B10, MacPNS1, MacPNS2 X1.1, PHP-EC, PHP-AX, PHP-eB PHP-S, learn more by clicking the button below!