We can provide our Center members with access to imaging instruments through close collaborations with Dr. Xu’s lab.
- We have a new Olympus VS120 system, which is designed for high throughput imaging of brain slices. Its slide loader system allows for loading up to six standard slides for fast and high resolution scanning.
- There are two Olympus research microscopes equipped with fluorescence optics and high-sensitive CCD cameras. The systems are equipped with commercial and custom-designed software for brain-wide analysis of genetically labeled tissue.
- The Xu lab has a state-of-the-art multiphoton imaging system: Resonant scanning, fast-sampling (125 MS/s), multiphoton microscope, two GaAsP PMTs, inertia-less, fast Z-focus with integrated spherical treadmill and pupil monitoring (Neurolabware); dual output, broadly tunable laser (660 nm to 1320 nm) for multiphoton imaging (Chameleon Discovery with TOTALPOWER CONTROL, Coherent Inc.)
- The Xu lab has a number of miniature fluorescent microscopes and related data acquisition systems. These miniscopes are very useful for in vivo calcium imaging of neural ensemble activity in freely behaving animals. As part of an existing collaboration, we have dependable access to imaging resources and technical expertise offered by Dr. Peyman Golshani’s group who has developed the UCLA miniscope system (www.miniscope.org/).
Illustration depicts immunofluorescent staining of anti-β-amyloid, 1-16 antibody against 3x-Tg and non-transgenic mouse tissue at dorsal CA1 at two time points. Non-transgenic mice showed no visible deposition of β amyloid at 7 months and 22 months of age. Meanwhile, 3x-Tg mice showed early intracellular deposition at 7 months in pyramidal layer and rapid manifestation of extracellular amyloid plaques at 22 months of age. Images captured from Olympus VS120-S6 system at 10X magnification.
Dil-tracing of CA1 and subiculum connections on post-mortem hippocampus samples from Alzheimer’s and mildly cognitive impaired patients. Images captured from Olympus VS120-S6 system at 10X magnification.
Illustration shows series of immunofluorescent staining against a wide variety of neurochemical proteins to highlight select populations of neurons and glial cells in the pigtail macaque hippocampus. The neurochemical markers include NeuN, parvalbumin (PV), GABA, CamK2A, somatostatin (SST), vasoactive intestinal peptide (VIP), glial fibrillary acidic protein (GFAP), and Iba-1. Images captured from Olympus VS120-S6 system at 10X magnification.
Illustration shows bright field (BF) imaging of immunohistochemistry (IHC) staining results against h-Tau7 proteins, a hallmark biomarker of Alzheimer’s disease, in 3x-Tg and non-transgenic control mice at two time points in dorsal CA1 portion of hippocampus. Significant presence of hTau-7 staining can be seen in 3x-Tg mice at 7months and 22months of age. Images captured from Olympus VS120-S6 system at 10X magnification.