Our new recombinant Rabies Virus (RV) tools for multi-scale imaging express a range of improved fluorescent proteins. We have developed 20 new RV vectors for multi-scale and multi-modal neural circuit mapping tools. We have refined the virus to target specific neuronal subcellular locations of interest. These tools can also be used in EM and in vivo imaging.
All viruses are propagated on B7GG cells, concentrated and purified via ultra-centrifugation. Titers are validated via plaque assay and meet our titer of 108 PFU/mL (plaque forming units per mL).
More information about our Rabies viruses can be found on the following publication: New rabies viral resources for multi-scale neural circuit mapping. Alexis Bouin, Ginny Wu, et al. Mol Psychiatry (2024). https://doi.org/10.1038/s41380-024-02451-6
G-Deleted Rabies Virus
All G-Deleted Rabies viruses are propagated on B7GG cells and purified via ultracentrifugation. Titers are validated via plaque assay and meet our titer of 108 PFU/mL (plaque forming units per mL). Sold as a 10ul aliquot.
We plan to offer both SAD-B19 and CVS-CN2C strains of recombinant RVΔG. Please contact us if you’d order the CVS-N2C version of RVΔG.
| Catalogue# | Name | Reporter | Color | Localization |
|---|---|---|---|---|
| RVdG-1 | SAD-B19-RVΔG EBFP | EBFP | Blue | Cytoplasm |
| RVdG-2 | SAD-B19-RVΔG mTurquoise2 | mTurquoise2 | Blue/green | Cytoplasm |
| RVdG-3 | SAD-B19-RVΔG mTFP1 | mTFP1 | Blue/green | Cytoplasm |
| RVdG-4 | SAD-B19-RVΔG mNeonGreen | mNeonGreen | Green | Cytoplasm |
| RVdG-5 | SAD-B19-RVΔG tdTomato | tdTomato | Red | Cytoplasm |
| RVdG-6 | SAD-B19-RVΔG smURFP | smURFP | Far Red | Cytoplasm |
| RVdG-7 | SAD-B19-RVΔG Slow-FT (blue / red) | Slow FT | Switch blue to red | Cytoplasm |
| RVdG-8 | SAD-B19-RVΔG PSD95-mNeonGreen | mNeonGreen | Green | Post synaptic |
| RVdG-9 | SAD-B19-RVΔG SNPN-tdTomato | tdTomato | Red | Presynaptic |
| RVdG-10 | SAD-B19-RVΔG ChR2-EYFP-Kv | EYFP | Yellow | Soma |
| RVdG-11 | SAD-B19-RVΔG 1XNLS-tdTomato | tdTomato | Red | Nucleus |
| RVdG-12 | SAD-B19-RVΔG 2XNLS-tdTomato | tdTomato | Red | Nucleus |
| RVdG-13 | SAD-B19-RVΔG MLS-tdTomato | tdTomato | Red | Membrane |
| RVdG-14 | SAD-B19-RVΔG 2XMito-dTomato | tdTomato | Red | Mitochondria |
| RVdG-15 | SAD-B19-RVΔG mNeonGreen-T2A-2XMito-dTomato | mNeonGreen / dTomato | Green/ Red | Cytoplasm / Mitochondria |
| RVdG-16 | SAD-B19-RVΔG tdTomato-T2A-Nluc | tdTomato / Nluc | Red | Cytoplasm |
| RVdG-17 | SAD-B19-RVΔG EGFP-FTL (ferritin) | EGPF | Green | Cytoplasmic aggregates |
| RVdG-18 | SAD-B19-RVΔG emGFP-T2A-FTL (ferritin) | emGFP | Green | Cytoplasm |
| RVdG-19 | SAD-B19-RVΔG emGFP-T2A-MLS-FTL (ferritin) | emGFP | Green | Cytoplasm |
| RVdG-20 | SAD-B19-RVΔG GCaMP7f | GCaMP7f | Green | Cytoplasm |
| RVdG-21 | SAD-B19-RVΔG -iRFP | iRFP | Far red | Cytoplasm |
EnvA Pseudotyped G-deleted Rabies Virus
EnvA-pseudotyped recombinant rabies cannot infect mammalian cells but it will infect only the cells that express exogenous receptor TVA on it surface (starter cells).
All EnvA Pseudotyped G-Deleted Rabies viruses are propagated on EnvA-B7GG cells and purified via ultracentrifugation. Titers are validated via plaque assay and meet our titer of 108 PFU/mL (plaque forming units per mL). Sold as a 10ul aliquot.
| Catalogue# | Name | Reporter | Color | Localization |
|---|---|---|---|---|
| EnvA-RV-1 | EnvA-SAD-B19-RVΔG mNeonGreen | mNeonGreen | Green | Cytoplasm |
| EnvA-RV-2 | EnvA-SAD-B19-RVΔG tdTomato | tdTomato | Red | Cytoplasm |
| EnvA-RV-3 | EnvA-SAD-B19-RVΔG 2XNLS-tdTomato | tdTomato | Red | Nucleus |
| EnvA-RV-4 | EnvA-SAD-B19-RVΔG EGFP-FTL (ferritin) | EGPF | Green | Cytoplasmic aggregates |
| EnvA-RV-5 | EnvA-SAD-B19-RVΔG emGFP-T2A-FTL (ferritin) | emGFP | Green | Cytoplasm |
| EnvA-RV-6 | EnvA-SAD-B19-RVΔG emGFP-T2A-MLS-FTL (ferritin) | emGFP | Green | Cytoplasm |
| EnvA-RV-7 | EnvA-SAD-B19-RVΔG GCaMP7f | GCaMP7f | Green | Cytoplasm |
Helper AAVs
AAVs are produced in AAV-ProHek293T cells and purified via iodixanol gradient and ultracentrifugation. Titers are validated via digital droplet PCR (ddPCR) and meet our titer of 1×10 13 GC/mL (genome copies per mL). Helper AAVs are validated with the AAV8 serotype. Sold as a 50ul aliquot. If you require another serotype it will be a full size prep of 0.5mL.
| Catalogue# | Name | Virus Description |
| AAV-RV-1 | AAV8-hSyn-TC66T-2A-eGFP-2A-OG | Expresses TVA (TC66T), eGFP, and optimized Rabies G (oG) protein |
| AAV-RV-2 | AAV8-hSyn-TC66T-2A-mRuby3-2A-OG | Expresses TVA (TC66T), mRuby, and optimized Rabies G (oG) protein |
| AAV-RV-3 | AAV8-DIO-hSyn-TC66T-2A-eGFP-2A-OG | Expresses TVA (TC66T), eGFP, and optimized Rabies G (oG) protein in a FLEX cassette |
| AAV-RV-4 | AAV8-DIO-hSyn-TC66T-2A-mRuby3-2A-OG | Expresses TVA (TC66T), mRuby, and optimized Rabies G (oG) protein in a FLEX cassette |
| AAV-RV-5 | AAV8-S5E2-TC66T-P2A-GFP-P2A- OG | Expresses TVA (TC66T), targeting parvalbumin (PV) inhibitory neurons, and optimized Rabies G (oG) protein |