Viral Production Facility

Our viral production facility aims at aiding investigators in the development and production of viral vectors for in-vivo and in-vitro experimentation purposes. Our state-of-the-art equipment coupled with coupled with our expertise allows use to ensure high quality products. We will provide all related sequence data, vector maps, plasmids and ready-to-use viruses once your orders are completed. Our facility has an UC approved, recharge-based service platform and we accept both UC and external orders.

Example data from monosynaptic rabies virus tracing from mouse hippocamal CA. The image of a mouse coronal brain section shows starter neurons in the dorsal hippocampal CA1, labeled by both eGFP and dsRed expression from both AAV and rabies virus infection. Their presynaptic partners (for example, local interneurons and CA3 neurons) are labeled by red fluorescent protein dsRed from rabies virus infection. See more information at Sun et al. (2019, Nature Neurosci).

Virus Types

Select your virus type of interest below.

AAV-Based GRAB Sensors* (click here to see list) (Relevant article) – AAV1, AAV9, AAV-PHP.eB only, 100ul preps.

Testing, Validation, and Training Services

We offer testing, validation and training services for your lab’s need.

Service & DescriptionUCsnon-UCs
In-vivo Virus Injection & Scan
-Perfect for test pilot experiments or lab lacking infrastructure
-Cost includes: animal housing, feeding, virus injection, sacrifice, perfusion, cut & scan
-Must provide: animal, additional antibodies
-Must purchase virus from CNCM depository or custom AAV
$1,339.11$2,000.00
Surgery and Perfusion Training
-Perfect for labs considering setting up a new protocol
-Perfect for new trainees, junior scientists
-Surgery, virus injection, sacrifice and perfusion training
$896.07$1,500.00
Silver Stain and Endotoxin Screen
-Validation of AAV purity
-Must purchase virus from CNCM.
$373.00 $500.00
FAQs

What is the expected turnaround time for out of stock items? Please allow 7 weeks for out of stock items to be made and validated.

How to I obtain a vector to package into an AAV virus? Some plasmids are available via Addgene and can shipped directly to our lab. We also have custom vector building service to build your construct of interest.

Are there packaging limits for AAVs? The packaging limit for AAvs is 5 Kb. Any larger vectors may require more starting material and higher costs; if we are unable to fulfill your custom order we reserve the right to cancel it at any time.

Do you offer custom Rabies, Herpes (H129), Yellow Fever Virus (YFV), Lenti virus services? These are offered on an case by case basis. Please fill out the investigator form and we will put together a custom quote for you.

Do you offer plasmids or plasmid maps? No, we do not provide any plasmids or plasmid maps. Exception: custom AAV services which starting material is provided by the customer or synthesized for them.

What documentation is required for obtaining virus? We require proof of an working IBC and IACUC (if being used as injection into live animals) protocols.

What will be included in our shipment? Your virus will be shipped or dropped off on dry ice. They will include a data sheet with titer and handling directions. Please allow the CNCM staff to handle all logistics for shipping and handling, including delivery on UCI campus.

I am an external institution, what other items are required? We require a sales & service contract, our staff will handle the point of sale with your institution.

Terms and conditions? For all depository items we own the intellectual and distribution rights to. Any modifications to our viruses must be obtained in writing and will be reviewed on case-by-case basis. We require all items that are used in publications to acknowledge the UCI Center for Neural Circuit Mapping (CNCM) Viral Core.

What payment forms do you accept? For UCs we take account/fund numbers and bill you internally. For external institutions, we accept check and bank wire. Before we deliver we request a PO or copy of order from your institution.

Contact Us / Order
CNCM Viral Core Order
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